Cryo-electron microscopy for high-resolution protein reconstruction
Enhance your protein structural analysis with cutting-edge cryo-electron microscopy, designed for precise high-throughput image acquisition and 3D reconstruction, crucial for advancing drug discovery and biomolecular research.
The Tundra Cryo-TEM from Thermo Fisher Scientific is a specialized cryo-electron microscope designed to unveil intricate biomolecular structures, crucial for pharmaceutical and biotechnology research. Equipped with the Falcon C Direct Electron Detector, it leverages high detective quantum efficiency for precise protein image acquisition and reconstruction. With applications spanning biopharmaceuticals and structural biology, it excels in processing protein complexes for drug discovery and cryo-EM studies. The Tundra Cryo-TEM integrates automation and high-throughput capabilities, allowing seamless 3D reconstruction, motion correction, and structure analysis. Its automation is enhanced by AI-driven software, ensuring optimal operation and reducing manual intervention. Ideal for academic and industrial labs, it supports critical research with efficient data management and electron counting, promising reliable and repeatable results for demanding scientific inquiries.
Benefits
- Enhances research productivity with high-resolution imaging and rapid data acquisition.
- Streamlines complex workflows with integrated AI-driven automation, reducing manual oversight.
- Delivers precise structural insights, crucial for drug discovery and protein analysis.
- Minimizes data storage requirements with efficient lossless data compression.
- Supports diverse biopharmaceutical and structural biology applications with versatile integration options.
- Applications
- Cryo-electron microscopy,Proteins,Drug discovery,Biopharmaceuticals,Cell and gene therapy,Structural biology
- End products
- Transthyretin receptor structures,Cryo-tem derived structural models of biomolecules,T20s proteasome structures,Apoferritin structures,Protein reconstructions in cryo-em studies,Protein complexes for drug discovery
- Steps before
- Sample preparation,Protein extraction,Cryo-EM grid preparation,Vitreous ice embedding
- Steps after
- Data processing,3D reconstruction,Analysis of protein structure,Single particle analysis,Cryo-electron tomography
- Input ingredients
- biological samples,proteins embedded in vitreous ice,electron events,protein structures,single particle analysis,cryo-electron tomography
- Output ingredients
- high-resolution structures,3D reconstructions,electron-event representation data,protein structures,cryo-EM data,single particle data,cellular structural analysis
- Operating voltage
- 100 kV
- Camera architecture
- Direct electron detection
- Sensor size
- 2,048 x 2,048 pixels,5.7 x 5.7 cm²
- Pixel size
- 28 x 28 μm²
- Internal frame rate
- 250 fps
- Frame rate to storage
- 240 fps (EER mode)
- Camera overhead time
- 0.5 s per acquisition
- File formats
- EER (native),MRC as integrated image
- Detection modes
- Electron counting mode,Survey mode
- Imaging performance in EER mode
- DQE(0) ≥ 0.95,DQE(½ Nyquist) ≥ 0.55,DQE(1 Nyquist) ≥ 0.2
- Automation level
- User-friendly operation with AI-driven plugins
- Data acquisition speed
- High throughput data collection
- Image acquisition techniques
- Electron counting and survey imaging modes
- Data storage format
- Lossless data compression (EER)
- Image processing capability
- Integrated image quality improvement during 3D reconstruction
- Sample drift correction
- Motion correction in dose-fractions
- Beam shift technology
- Aberration-free image shift (AFIS)
- Density/particle size
- 0.5–2.5 g/cm³ / 50–1000 µm
- Machine footprint
- Compact
- Camera architecture
- Direct electron detection
- Sensor size
- 2,048 x 2,048 pixels,5.7 x 5.7 cm²
- Pixel size
- 28 x 28 μm²
- TEM Operating voltage
- 100 kV
- Internal frame rate
- 250 fps
- Frame rate to storage
- 240 fps (EER mode)
- Camera overhead time
- 0.5 s per acquisition
- Compact footprint
- Custom configurations available
- Camera type
- Direct electron detection / CMOS
- Detector type
- Falcon C Direct Electron Detector / Ceta F CMOS Camera
- Integration software
- Tundra software / EPU / Smart EPU
- Resolution options
- High resolution / Standard resolution
- Storage options
- EER mode / MRC as integrated image
- Imaging mode
- Electron counting mode / Survey mode
- Internal frame rate
- 250 fps / 240 fps (EER mode)
- Automation
- Smart EPU Software / AI-enabled
- Pixel size
- 28 x 28 μm²
- Market info
- Thermo Fisher Scientific is renowned for providing innovative scientific instruments, laboratory equipment, and engineered-to-order solutions across various industries, esteemed for their high-quality products and expertise in life sciences, pharmaceuticals, and biotechnology sectors.