Cultivation system for microtiter plates
Accelerate your strain cultivation process with a micro-bioreactor system that enables the simultaneous testing and optimization of microbial growth conditions with minimal handling.
The Applikon Micro-Flask from Getinge is designed for efficient cultivation and screening of microbial strains and cell lines. It employs individual micro-bioreactors within microtiter plates to generate reproducible results in high-throughput settings. This system is invaluable for biopharmaceuticals and biotechnology research, enabling rapid testing of recombinant proteins, monoclonal antibodies, and novel antibiotics. The Micro-Flask system offers oxygen transfer rates comparable to traditional shake flasks, enhancing culture quality while reducing processing time by up to tenfold. Its compatibility with various microtiter plate formats makes it versatile across applications, such as enzyme formulation and microbial cultivation. With minimal cross-contamination, it integrates seamlessly into R&D and laboratory workflows, supporting manual and high-throughput operations.
Benefits
- Increases screening capacity, allowing simultaneous cultivation of thousands of samples.
- Reduces manual labor with integrated parallel handling of multiple strains.
- Ensures consistent and reproducible results with optimized oxygen transfer rates.
- Minimizes cross-contamination with sterile barriers for individual wells.
- Adapts easily to varied production needs with compatibility across multiple microtiter plate formats.
- Applications
- Microbial strains,Secondary metabolites,Mutant banks,Biopharmaceuticals,Enzymes,Clonal libraries,Cell lines
- End products
- Genetically modified organisms,Monoclonal antibodies,Bioactive compounds,Enzyme formulations,Industrial microbes,Novel antibiotics,Recombinant proteins
- Steps before
- Preparation of microbial strains,Loading of microtiter plates,Cultivation setup
- Steps after
- Analysis of growth results,Screening for high-activity mutants,Optimization studies
- Input ingredients
- microbial strains,clonal libraries,mutant banks,cell lines,growth medium,microtiter plates
- Output ingredients
- cultivated microbial strains,high-activity mutants,high-productivity mutants,new enzyme activities,formation of secondary metabolites
- Well volume
- 11000 µL,3000 µL,2400 µL,380 µL
- Culture volume
- 2500 µL,750 µL,500 µL,100 µL
- Orbital shaking frequency
- 300 rpm,220 rpm
- Shaking amplitude
- 25 mm,50 mm
- O2-transfer rate
- 51 mmol O2/l/h,39 mmol O2/l/h,24 mmol O2/l/h
- Headspace refreshment rate
- 2.5 mL/min,1.1 mL/min,1 mL/min,250 µL/min
- Evaporation rate per well
- 50 µL H2O per day,25 µL H2O per day,30 µL H2O per day,15 µL H2O per day,22 µL H2O per day,11 µL H2O per day,6 µL H2O per day,3 µL H2O per day
- Batch vs. continuous operation
- Simultaneous batch processing
- Automated handling
- Parallel handling with Cryo-Replicator
- Cross-contamination prevention
- Sterile barrier for individual wells
- Oxygen transfer
- Similar to shake flasks
- Evaporation control
- Low and uniform evaporation rates
- Density/particle size
- 0.5–2.5 g/cm³ / 50–1000 µm
- Footprint
- Compact design for lab spaces
- Microtiter plate sizes supported
- 6,24,96-well plates
- Well volume range
- 100 µL - 2500 µL
- Material
- Polypropylene,Polystyrene
- Dimensions of deep-well plates
- 17x17 mm,depth 40 mm
- Dimensions of low-well plates
- ⌀16 mm,depth 18 mm
- Clamp mechanism
- Stainless steel cover with silicone seal
- Headspace management
- ePTFE + microfiber layer
- Cryo replicator compatibility
- Yes
- Well Type
- Deep-well / Low-well
- Plate Material
- Polypropylene / Polystyrene
- Headspace Refreshment Rate
- 1-2.5 mL / min
- Oxygen Transfer Rate
- 12-51 mmol O2/l/h
- Control Panel Type
- Manual
- Clamp Type
- Stainless steel cover
- Cover Type
- Sandwich-cover (silicone seal,ePTFE + microfibre layer)
- Market info
- Getinge is known for providing innovative healthcare and life sciences products, specializing in medical technology, infection control, surgical workflows, and critical care solutions. They maintain a strong reputation for quality and technological advancement in these sectors.